Regulation and Characterization of Amylase Enzyme Secretion In the Digestive Tract of <i>Antheraea Assamensis</i> Helfer

Antheraea assamensis Helfer is an endemic non-mulberry lepidopteran species of North East India with high commercial demand for its golden hued silk. suffers from a protozoan disease pebrine which lead to death the silkworm. In this study, effect various factors (starvation, feeding, temperature, pH, Ca ion and larval stage) in regulation amylase secretion compared healthy diseased muga Moreover, enzyme zymography purification midgut also performed. Result shows that, starved condition, significantly decreases. Refeeding experiments (after 2 days starvation) suggest that sharply increases due feeding stimulus. The purified molecular weight confirmed as 56 kDa by SDS PAGE. fold 34 times higher than crude extract.